[1]苗玉发,康慧君,王 超,等.血清维生素D结合蛋白水平检测对大鼠肝毒性诊断的评价研究[J].现代检验医学杂志,2019,34(04):6-10.[doi:10.3969/j.issn.1671-7414.2019.04.002]
 MIAO Yu-fa,KANG Hui-jun,WANG Chao,et al.Evaluation of Serum Vitamin D Binding Protein Levels in the Diagnosis of Hepatotoxicity in Rats[J].Journal of Modern Laboratory Medicine,2019,34(04):6-10.[doi:10.3969/j.issn.1671-7414.2019.04.002]
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血清维生素D结合蛋白水平检测对大鼠肝毒性诊断的评价研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年04期
页码:
6-10
栏目:
论 著
出版日期:
2019-08-20

文章信息/Info

Title:
Evaluation of Serum Vitamin D Binding Protein Levels in the Diagnosis of Hepatotoxicity in Rats
文章编号:
1671-7414(2019)04-006-05
作者:
苗玉发康慧君王 超张河战
(中国食品药品检定研究院,药物非临床安全评价研究北京市重点实验室,北京 100176)
Author(s):
MIAO Yu-faKANG Hui-junWANG ChaoZHANG He-zhan
(National Institutes for Food and Drug Control, Beijing Key Laboratory for Safety Evaluation of Drug,Beijing 100176,China)
关键词:
维生素D结合蛋白 肝功能 肝毒性
分类号:
R-332
DOI:
10.3969/j.issn.1671-7414.2019.04.002
文献标志码:
A
摘要:
目的 评价血清维生素D结合蛋白(vitamin D binding protein,VDBP)用于大鼠肝毒性诊断的价值。方法 用酮康唑(ketoconazole,KTZ)、四氯化碳(carbon tetrachloride,CCl4)和对乙酰氨基酚(acetaminophen,APAP)在雄性大鼠中构建3种肝毒性模型,在不同时间点从腹腔大静脉采集外周血,3 000 r/min离心制备血清。用ELISA法测定血清中VDBP含量,用全自动生化分析仪测定血清中的丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)、碱性磷酸酶(alkaline phosphatase,ALP)、总胆红素(total bilirubin,TBIL)、总胆汁酸(total biliary acid,TBA)、胆碱酯酶(cholinesterase,CHE)、谷氨酸脱氢酶(glutamate dehydrogenase,GLDH)、5'核苷酸酶(5'-nucleoticlase,5'-NT)、单胺氧化酶(monoamine oxidase,MAO)和苹果酸脱氢酶(malate dehydrogenase,MDH)含量。实验组与对照组进行t检验分析,检查指标值的差异是否具有统计学意义。结果 在CCl4肝毒性模型中,与对照组相比,ALT,AST,TBIL和CHE在CCl4给药后4 h起升高,差异均具有统计学意义(均P<0.05)。GLDH,MAO和MDH在给药后6 h起升高,差异均具有统计学意义(均P<0.05)。在KTZ肝毒性模型中,与对照组相比,ALT在KTZ给药6天后升高,差异具有统计学意义(P<0.05)。AST和TBIL在给药后8天升高,差异具有统计学意义(P<0.05)。在APAP肝毒性模型中,与对照组相比,ALP在APAP给药后4 h和8 h点升高,差异具有统计学意义(P<0.05)。CHE在给药后8 h点升高,差异具有统计学意义(P<0.05)。ALT和TBIL在给药后24 h点升高,差异具有统计学意义(P<0.05)。在3种肝毒性模型中,VDBP含量在给药后的所有时间点都降低,差异均具有统计学意义(均P<0.05)。结论 VDBP比其它肝功能指标能更灵敏地预测肝毒性的发生。

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备注/Memo

备注/Memo:
基金项目:国家“重大新药创制”科技重大专项(2018ZX09201017)。 作者简介:苗玉发(1980-),男,硕士,副主任药师,主要研究方向为药物安全性评价,E-mail:miaoyufa@nifdc.org.cn。 通讯作者:张河战,研究员,E-mail:zhanghz@nifdc.org.cn。收稿日期:2019-05-14 修回日期:2019-05-17
更新日期/Last Update: 2019-07-30